Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.

ABSTRACT: Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the embryo uncertain. Here, we avoided using feeder cells or serum factors to provide a defined culture microenvironment. We show that the combination of activin A, fibroblast growth factor and the Wnt inhibitor XAV939 (AFX) supports establishment and continuous expansion of pluripotent stem cell lines from porcine, ovine and bovine embryos. Germ layer differentiation was evident in teratomas and readily induced in vitro. Global transcriptome analyses highlighted commonality in transcription factor expression across the three species, while global co

SUBMITTER: Kinoshita M 

PROVIDER: S-EPMC8714072 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature