Rewards of divergence in sequences, 3-D structures and dynamics of yeast and human spliceosome SF3b complexes.

ABSTRACT: The evolution of homologous and functionally equivalent multiprotein assemblies is intriguing considering sequence divergence of constituent proteins. Here, we studied the implications of protein sequence divergence on the structure, dynamics and function of homologous yeast and human SF3b spliceosomal subcomplexes. Human and yeast SF3b comprise of 7 and 6 proteins respectively, with all yeast proteins homologous to their human counterparts at moderate sequence identity. SF3b6, an additional component in the human SF3b, interacts with the N-terminal extension of SF3b1 while the yeast homologue Hsh155 lacks the equivalent region. Through detailed homology studies, we show that SF3b6 is absent not only in yeast but in multiple lineages of eukaryotes implying that it is critical in specific o

SUBMITTER: Yazhini A 

PROVIDER: S-EPMC8714771 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature