Levulinic Acid-Inducible and Tunable Gene Expression System for Methylorubrum extorquens.

ABSTRACT: Methylorubrum extorquens AM1 is an efficient platform strain possessing biotechnological potential in formate- and methanol-based single carbon (C1) bioeconomy. Constitutive expression or costly chemical-inducible expression systems are not always desirable. Here, several glucose-, xylose-, and levulinic acid (LA)-inducible promoter systems were assessed for the induction of green fluorescent protein (GFP) as a reporter protein. Among them, the LA-inducible gene expression system (HpdR/P _hpdH ) showed a strong expression of GFP (51-fold) compared to the control. The system was induced even at a low concentration of LA (0.1 mM). The fluorescence intensity increased with increasing concentrations of LA up to 20 mM. The system was tunable and tightly controlled with m

SUBMITTER: Sathesh-Prabu C 

PROVIDER: S-EPMC8714952 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature