Few-Photon Spectral Confocal Microscopy for Cell Imaging Using Superconducting Transition Edge Sensor.

ABSTRACT: A superconducting transition edge sensor (TES) is an energy-dispersive single-photon detector that distinguishes the wavelength of each incident photon from visible to near-infrared (NIR) without using spectral dispersive elements. Here, we introduce an application of the TES technique for confocal laser scanning microscopy (CLSM) as proof of our concept of ultra-sensitive and wide-band wavelength range color imaging for biological samples. As a reference sample for wide-band observation, a fixed fluorescence-labeled cell sample stained with three different color dyes was observed using our TES-based CLSM method. The three different dyes were simultaneously excited by irradiating 405 and 488 nm lasers, which were coupled using an optical fiber combiner. Even when irradiated at low powers o

SUBMITTER: Niwa K 

PROVIDER: S-EPMC8715037 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature