Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy.

ABSTRACT: Life science research often needs to define where molecules are located within the complex environment of a cell or tissue. Genetically encoded fluorescent proteins and or fluorescence affinity-labeling are the go-to methods. Although recent fluorescent microscopy methods can provide localization of fluorescent molecules with relatively high resolution, an ultrastructural context is missing. This is solved by imaging a region of interest with correlative light and electron microscopy (CLEM). We have adopted a protocol that preserves both genetically-encoded and antibody-derived fluorescent signals in resin-embedded cell and tissue samples and provides high-resolution electron microscopy imaging of the same thin section. This method is particularly suitable for dedicated CLEM instruments th

SUBMITTER: Baatsen P 

PROVIDER: S-EPMC8715528 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature