SERSbot: Revealing the Details of SERS Multianalyte Sensing Using Full Automation.

ABSTRACT: Surface-enhanced Raman spectroscopy (SERS) is considered an attractive candidate for quantitative and multiplexed molecular sensing of analytes whose chemical composition is not fully known. In principle, molecules can be identified through their fingerprint spectrum when binding inside plasmonic hotspots. However, competitive binding experiments between methyl viologen (MV²⁺) and its deuterated isomer (d₈-MV²⁺) here show that determining individual concentrations by extracting peak intensities from spectra is not possible. This is because analytes bind to different binding sites inside and outside of hotspots with different affinities. Only by knowing all binding constants and geometry-related factors, can a model revealing accurate concentrations b

SUBMITTER: Grys DB 

PROVIDER: S-EPMC8715530 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature