Astrocytic C-X-C motif chemokine ligand-1 mediates β-amyloid-induced synaptotoxicity.

ABSTRACT:

Background

Pathological interactions between β-amyloid (Aβ) and tau drive synapse loss and cognitive decline in Alzheimer's disease (AD). Reactive astrocytes, displaying altered functions, are also a prominent feature of AD brain. This large and heterogeneous population of cells are increasingly recognised as contributing to early phases of disease. However, the contribution of astrocytes to Aβ-induced synaptotoxicity in AD is not well understood.

Methods

We stimulated mouse and human astrocytes with conditioned medium containing concentrations and species of human Aβ that mimic those in human AD brain. Medium from stimulated astrocytes was collected and immunodepleted of Aβ before being added to naïve rodent or human neuron cultures. A cytokine, identified in unbiased scree

SUBMITTER: Perez-Nievas BG 

PROVIDER: S-EPMC8715604 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature