Linezolid resistance in Mycobacterium tuberculosis isolates at a tertiary care centre in Mumbai, India.

ABSTRACT:

Background & objectives

Linezolid (LZD) is increasingly being used in tuberculosis (TB) treatment. However, LZD resistance has already been reported, which is highly alarming, given its critical therapeutic role. This study was aimed to phenotypically and genotypically assess LZD resistance in Mycobacterium tuberculosis (MTB) isolates at a laboratory in a tertiary care centre in Mumbai, India.

Methods

A sample of 32 consecutive LZD-resistant MTB isolates identified by liquid culture susceptibility testing was subjected to whole-genome sequencing (WGS) on the Illumina NextSeq platform. Sequences were analyzed using BioNumerics software to predict resistance for 12 antibiotics within 15 min.

Results

Sixty eight of the 2179 isolates tested for LZD resistance by MGIT-base

SUBMITTER: Nambiar R 

PROVIDER: S-EPMC8715694 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature