An LC-MS/MS assay and complementary web-based tool to quantify and predict compound accumulation in E. coli.

ABSTRACT: Novel classes of broad-spectrum antibiotics have been extremely difficult to discover, largely due to the impermeability of the Gram-negative membranes coupled with a poor understanding of the physicochemical properties a compound should possess to promote its accumulation inside the cell. To address this challenge, numerous methodologies for assessing intracellular compound accumulation in Gram-negative bacteria have been established, including classic radiometric and fluorescence-based methods. The recent development of accumulation assays that utilize liquid chromatography-tandem mass spectrometry (LC-MS/MS) have circumvented the requirement for labeled compounds, enabling assessment of a substantially broader range of small molecules. Our unbiased study of accumulation trends in Escher

SUBMITTER: Geddes EJ 

PROVIDER: S-EPMC8715754 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature