Single-cell transcriptome atlas of human mesenchymal stem cells exploring cellular heterogeneity.

ABSTRACT:

Background

The heterogeneity of mesenchymal stem cells (MSCs) is poorly understood, thus limiting clinical application and basic research reproducibility. Advanced single-cell RNA sequencing (scRNA-seq) is a robust tool used to analyse for dissecting cellular heterogeneity. However, the comprehensive single-cell atlas for human MSCs has not been achieved.

Methods

This study used massive parallel multiplexing scRNA-seq to construct an atlas of > 130 000 single-MSC transcriptomes across multiple tissues and donors to assess their heterogeneity. The most widely clinically utilised tissue resources for MSCs were collected, including normal bone marrow (n = 3), adipose (n = 3), umbilical cord (n = 2), and dermis (n = 3).

Results

Seven tissue-specific and five conserved MSC

SUBMITTER: Wang Z 

PROVIDER: S-EPMC8715893 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature