Blastocyst development after fertilization with in vitro spermatids derived from nonhuman primate embryonic stem cells.

ABSTRACT:

Objective

To demonstrate that functional spermatids can be derived in vitro from nonhuman primate pluripotent stem cells.

Design

Green fluorescent protein-labeled, rhesus macaque nonhuman primate embryonic stem cells (nhpESCs) were differentiated into advanced male germ cell lineages using a modified serum-free spermatogonial stem cell culture medium. In vitro-derived round spermatid-like cells (rSLCs) from differentiated nhpESCs were assessed for their ability to fertilize rhesus oocytes by intracytoplasmic sperm(atid) injection.

Setting

Multiple academic laboratory settings.

Patients

Not applicable.

Interventions

Intracytoplasmic sperm(atid) injection of in vitro-derived spermatids from nhpESCs into rhesus macaque oocytes.

Main outcome measures

SUBMITTER: Khampang S 

PROVIDER: S-EPMC8716017 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature