Macrophage tumor necrosis factor-alpha deletion does not protect against obesity-associated metabolic dysfunction.

ABSTRACT: The pro-inflammatory cytokine, tumor necrosis factor-alpha (TNF-α), has been suggested to be a key factor in the induction of obesity-associated metabolic dysfunction. However, the role that macrophage-derived TNF-α has on regulating metabolic perturbations in obesity is not completely understood. Therefore, we utilized the TNF-α^{Flox/Flox (F/F)} , LyzMcre^± mouse model to determine the impact that macrophage TNF-α deletion has on the development of high-fat diet (HFD)-induced obesity. At 10 weeks of age, male littermates were randomly assigned to 1 of 4 groups: TNF-α^F/F low-fat diet (TNF-α^F/F LFD), TNF-α^{F/F, LyzMCre} LFD, TNF-α^F/F HFD, or TNF-α^{F/F, LyzMCre} HFD (n = 16-28/group) and we

SUBMITTER: Aladhami AK 

PROVIDER: S-EPMC8716064 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature