Hepatitis B core antigen modulates exosomal miR-135a to target vesicle-associated membrane protein 2 promoting chemoresistance in hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. The association of hepatitis B virus (HBV) infection with HCC is hitherto documented. Exosomal miRNAs contribute to cancer progression and chemoresistance. HBV X protein has been known to modulate miRNAs that facilitate cell proliferation and the process of hepatocarcinogenesis. However, there has been no report on hepatitis B core antigen (HBc) regulating exosomal miRNAs to induce drug resistance of HCC cells.

Aim

To elucidate the mechanism by which HBc promotes Doxorubicin hydrochloride (Dox) resistance in HCC.

Methods

Exosomes were isolated by ultracentrifugation. The morphology and size of exosomes were evaluated by Dynamic Light Scattering (DLS) and transmission electron microsc

SUBMITTER: Wei XC 

PROVIDER: S-EPMC8717014 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature