Hydrogen sulfide regulates autophagy in nucleus pulposus cells under hypoxia.

ABSTRACT:

Objective

Hydrogen sulfide (H₂S) has been found to act as an important gasotransmitter to regulate cell activities. This study aimed to investigate the effect of H₂S on autophagy of nucleus pulposus (NP) cells under hypoxia and possible mechanism.

Materials and methods

NP cells were isolated from rat caudal discs. Cobalt chloride was used to mimic hypoxia, sodium hydrosulfide was used to emulate exogenous H₂S and 3-methyladenine was used to block cell autophagy. Cell viability was assessed by phase contrast microscope and Cell Counting Kit-8 method. Moreover, expression of key autophagic proteins was analyzed via western blotting, and transmission electron microscopy was performed to detect autophagosomes.

Results

Hypoxia markedly impa

SUBMITTER: Yue L 

PROVIDER: S-EPMC8717115 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature