On-Tissue Derivatization of Lipopolysaccharide for Detection of Lipid A Using MALDI-MSI.

ABSTRACT: We developed a method to directly detect and map the Gram-negative bacterial virulence factor lipid A derived from lipopolysaccharide (LPS) by coupling acid hydrolysis with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). As the structure of lipid A (endotoxin) determines the innate immune outcome during infection, the ability to map its location within an infected organ or animal is needed to understand localized inflammatory responses that results during host-pathogen interactions. We previously demonstrated detection of free lipid A from infected tissue; however detection of lipid A derived from intact (smooth) LPS from host-pathogen MSI studies, proved elusive. Here, we detected LPS-derived lipid A from the Gram-negative pathogens, Escherichia coli<

SUBMITTER: Yang H 

PROVIDER: S-EPMC8717242 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature