An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.

ABSTRACT: Despite their key phylogenetic position and their unique biology, hornworts have been widely overlooked. Until recently there was no hornwort model species amenable to systematic experimental investigation. Anthoceros agrestis has been proposed as the model species to study hornwort biology. We have developed an Agrobacterium-mediated method for the stable transformation of A. agrestis, a hornwort model species for which a genetic manipulation technique was not yet available. High transformation efficiency was achieved by using thallus tissue grown under low light conditions. We generated a total of 274 transgenic A. agrestis lines expressing the β-glucuronidase (GUS), cyan, green, and yellow fluorescent proteins under control of the CaMV 35S promoter and several endogenous promoters. Nucl

SUBMITTER: Frangedakis E 

PROVIDER: S-EPMC8717380 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature