Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones).

ABSTRACT: The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter-coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-performance liquid chromatography (RP-HPLC), polyacrylamide gel electrophoresis (PAGE), and nanoscale liquid chromatography coupled to mass spectrometry (nLC-MS/MS) analysis. Most of the fractions studied are hydrophobic, being proteins with molecular weights of ∼28, 62, and ∼198 kDa. These proteins seem to be homologous to proteins involved in product secretion, cytoskeleton, protein binding, cellular metabolism, and antimicrobial action among others. Lubricant function is also possible based on the literature.

SUBMITTER: Dos Santos Silva NF 

PROVIDER: S-EPMC8717388 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature