Minimal Pathway for the Regeneration of Redox Cofactors.

ABSTRACT: Effective metabolic pathways are essential for the construction of in vitro systems mimicking the biochemical complexity of living cells. Such pathways require the inclusion of a metabolic branch that ensures the availability of reducing equivalents. Here, we built a minimal enzymatic pathway confinable in the lumen of liposomes, in which the redox status of the nicotinamide cofactors NADH and NADPH is controlled by an externally provided formate. Formic acid permeates the membrane where a luminal formate dehydrogenase uses NAD⁺ to form NADH and carbon dioxide. Carbon dioxide diffuses out of the liposomes, leaving only the reducing equivalents in the lumen. A soluble transhydrogenase subsequently utilizes NADH for reduction of NADP⁺ thereby making NAD⁺ avai

SUBMITTER: Partipilo M 

PROVIDER: S-EPMC8717395 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature