Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model.

ABSTRACT: To develop effective adoptive cell transfer therapy using T cell receptor (TCR)-engineered T cells, it is critical to isolate tumor-reactive TCRs that have potent anti-tumor activity. In humans, tumor-infiltrating lymphocytes (TILs) have been reported to contain CD8⁺PD-1⁺ T cells that express tumor-reactive TCRs. Characterization of tumor reactivity of TILs from non-human primate tumors could improve anti-tumor activity of TCR-engineered T cells in preclinical research. In this study, we sought to isolate TCR genes from CD8⁺PD-1⁺ T cells among TILs in a cynomolgus macaque model of tumor transplantation in which the tumors were infiltrated with CD8⁺ T cells and were eventually rejected. We analyzed the repertoire of TCRα and β pairs obt

SUBMITTER: Terada K 

PROVIDER: S-EPMC8717465 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature