Determining the contributions of protein synthesis and breakdown to muscle atrophy requires non-steady-state equations.

ABSTRACT:

Background

Ageing and cachexia cause a loss of muscle mass over time, indicating that protein breakdown exceeds protein synthesis. Deuterium oxide (D₂ O) is used for studies of protein turnover because of the advantages of long-term labelling, but these methods introduce considerations that have been largely overlooked when studying conditions of protein gain or loss. The purpose of this study was to demonstrate the importance of accounting for a change in protein mass, a non-steady state, during D₂ O labelling studies while also exploring the contribution of protein synthesis and breakdown to denervation-induced muscle atrophy.

Methods

Adult (6 months) male C57BL/6 mice (n = 14) were labelled with D₂ O for a total of 7 days following unilate

SUBMITTER: Kobak KA 

PROVIDER: S-EPMC8718081 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature