H19X-encoded miR-322(424)/miR-503 regulates muscle mass by targeting translation initiation factors.

ABSTRACT:

Background

Skeletal muscle atrophy is a debilitating complication of many chronic diseases, disuse conditions, and ageing. Genome-wide gene expression analyses have identified that elevated levels of microRNAs encoded by the H19X locus are among the most significant changes in skeletal muscles in a wide scope of human cachectic conditions. We have previously reported that the H19X locus is important for the establishment of striated muscle fate during embryogenesis. However, the role of H19X-encoded microRNAs in regulating skeletal mass in adults is unknown.

Methods

We have created a transgenic mouse strain in which ectopic expression of miR-322/miR-503 is driven by the skeletal muscle-specific muscle creatine kinase promoter. We also used an H19X mutant mouse strain in whic

SUBMITTER: Liang R 

PROVIDER: S-EPMC8718088 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature