Functional independence of endogenous μ- and δ-opioid receptors co-expressed in cholinergic interneurons.

ABSTRACT: Class A G-protein-coupled receptors (GPCRs) normally function as monomers, although evidence from heterologous expression systems suggests that they may sometimes form homodimers and/or heterodimers. This study aims to evaluate possible functional interplay of endogenous µ- and δ-opioid receptors (MORs and DORs) in mouse neurons. Detecting GPCR dimers in native tissues, however, has been challenging. Previously, MORs and DORs co-expressed in transfected cells have been reported to form heterodimers, and their possible co-localization in neurons has been studied in knock-in mice expressing genetically engineered receptors fused to fluorescent proteins. Here, we find that single cholinergic neurons in the mouse striatum endogenously express both MORs and DORs. The receptors on neurons from l

SUBMITTER: Arttamangkul S 

PROVIDER: S-EPMC8718112 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature