HiCORE: Hi-C Analysis for Identification of Core Chromatin Looping Regions with Higher Resolution.

ABSTRACT: Genome-wide chromosome conformation capture (3C)- based high-throughput sequencing (Hi-C) has enabled identification of genome-wide chromatin loops. Because the Hi-C map with restriction fragment resolution is intrinsically associated with sparsity and stochastic noise, Hi-C data are usually binned at particular intervals; however, the binning method has limited reliability, especially at high resolution. Here, we describe a new method called HiCORE, which provides simple pipelines and algorithms to overcome the limitations of single-layered binning and predict core chromatin regions with three-dimensional physical interactions. In this approach, multiple layers of binning with slightly shifted genome coverage are generated, and interacting bins at each layer are integrated to infer narrow

SUBMITTER: Lee H 

PROVIDER: S-EPMC8718365 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature