A Ratiometric Calcium Reporter CGf Reveals Calcium Dynamics Both in the Single Cell and Whole Plant Levels Under Heat Stress.

ABSTRACT: Land plants evolved to quickly sense and adapt to temperature changes, such as hot days and cold nights. Given that calcium (Ca²⁺) signaling networks are implicated in most abiotic stress responses, heat-triggered changes in cytosolic Ca²⁺ were investigated in Arabidopsis leaves and pollen. Plants were engineered with a reporter called CGf, a ratiometric, genetically encoded Ca²⁺ reporter with an mCherry reference domain fused to an intensiometric Ca²⁺ reporter GCaMP6f. Relative changes in [Ca²⁺]_cyt were estimated based on CGf's apparent K _D around 220 nM. The ratiometric output provided an opportunity to compare Ca²⁺ dynamics between different tissues, cell types, or

SUBMITTER: Weigand C 

PROVIDER: S-EPMC8718611 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature