Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions.

ABSTRACT: Cell-free protein synthesis (CFPS) reactions have grown in popularity with particular interest in applications such as gene construct prototyping, biosensor technologies and the production of proteins with novel chemistry. Work has frequently focussed on optimising CFPS protocols for improving protein yield, reducing cost, or developing streamlined production protocols. Here we describe a statistical Design of Experiments analysis of 20 components of a popular CFPS reaction buffer. We simultaneously identify factors and factor interactions that impact on protein yield, rate of reaction, lag time and reaction longevity. This systematic experimental approach enables the creation of a statistical model capturing multiple behaviours of CFPS reactions in response to components and their interac

SUBMITTER: Banks AM 

PROVIDER: S-EPMC8718664 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature