Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO Cells.

ABSTRACT: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a β-coronavirus, is the causative agent of the COVID-19 pandemic. One of the three membrane-bound envelope proteins is the spike protein (S), the one responsible for docking to the cellular surface protein ACE2 enabling infection with SARS-CoV-2. Although the structure of the S-protein has distinct similarities to other viral envelope proteins, robust and straightforward protocols for recombinant expression and purification are not described in the literature. Therefore, most studies are done with truncated versions of the protein, like the receptor-binding domain. To learn more about the interaction of the virus with the ACE2 and other cell surface proteins, it is mandatory to provide recombinant spike protein in high struc

SUBMITTER: Mayrhofer P 

PROVIDER: S-EPMC8718689 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature