Targeted Gene Knockouts by Protoplast Transformation in the Moss Physcomitrella patens.

ABSTRACT: Targeted gene knockout is particularly useful for analyzing gene functions in plant growth, signaling, and development. By transforming knockout cassettes consisting of homologous sequences of the target gene into protoplasts, the classical gene targeting method aims to obtain targeted gene replacement, allowing for the characterization of gene functions in vivo. The moss Physcomitrella patens is a known model organism for a high frequency of homologous recombination and thus harbors a remarkable rate of gene targeting. Other moss features, including easy to culture, dominant haploidy phase, and sequenced genome, make gene targeting prevalent in Physcomitrella patens. However, even gene targeting was powerful to generate knockouts, researchers using this method still e

SUBMITTER: Zhu L 

PROVIDER: S-EPMC8718793 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature