Novel pentose-regulated promoter of Aspergillus oryzae with application in controlling heterologous gene expression.

ABSTRACT: The potent promoter and its transcriptional control make a significant contribution to strain optimization. Using transcriptome-based approach, a novel pentose-regulated promoter of the xylose reductase gene (PxyrA) of Aspergillus oryzae was identified. The promoter analysis showed that the PxyrA was tightly regulated by pentose sugars, which xylose and xylan were favorable inducers. The PxyrA function was highly efficient as compared with the maltose-inducible promoters of A. oryzae. It also exhibited the efficient transcription induction even though certain amounts of glucose and sucrose existed in the cultures. The expression control of PxyrA was dependent on xylose consumption capacity for fungal growth. The control mode of PxyrA 

SUBMITTER: Jeennor S 

PROVIDER: S-EPMC8718821 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature