VikAD, a Vika site-specific recombinase-based system for efficient and scalable helper-dependent adenovirus production.

ABSTRACT: Recombinant viral vectors have become integral tools for basic in vivo research applications. Helper-dependent adenoviral (HdAd) vectors have a large packaging capacity of ∼36 kb of DNA that mediate long-term transgene expression in vitro and in vivo. The large carrying capacity of HdAd enables basic research or clinical applications requiring the delivery of large genes or multiple transgenes, which cannot be packaged into other widely used viral vectors. Currently, common HdAd production systems use an Ad helper virus (HV) with a packaging signal (Ψ) that is flanked by either loxP or FRT sites, which is excised in producer cells expressing Cre or Flp recombinases to prevent HV packaging. However, these production systems prevent the use of HdAd vectors for gen

SUBMITTER: Phillips S 

PROVIDER: S-EPMC8718833 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature