Phylogeny and potential virulence of cryptic clade Escherichia coli species complex isolates derived from an arable field trial.

ABSTRACT: Analysis of Escherichia coli taxonomy has expanded into a species-complex with the identification of divergent cryptic clades. A key question is the evolutionary trajectory of these clades and their relationship to isolates of clinical or veterinary importance. Since they have some environmental association, we screened a collection of E. coli isolated from a long-term spring barley field trial for their presence. While most isolates clustered into the enteric-clade, four of them clustered into Clade-V, and one in Clade-IV. The Clade -V isolates shared >96% intra-clade average nucleotide sequence identity but <91% with other clades. Although pan-genomics analysis confirmed their taxonomy as Clade -V (E. marmotae), retrospective phylogroup PCR did not discriminate them correct

SUBMITTER: MacKenzie K 

PROVIDER: S-EPMC8718834 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature