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Ground tissue circuitry regulates organ complexity in maize and Setaria.

ABSTRACT: Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue to elaborate anatomical complexity.

SUBMITTER: Ortiz-Ramirez C

PROVIDER: S-EPMC8719420 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Ground tissue circuitry regulates organ complexity in maize and Setaria.

Ortiz-Ramírez Carlos C Guillotin Bruno B Xu Xiaosa X Rahni Ramin R Zhang Sanqiang S Yan Zhe Z Coqueiro Dias Araujo Poliana P Demesa-Arevalo Edgar E Lee Laura L Van Eck Joyce J Gingeras Thomas R TR Jackson David D Gallagher Kimberly L KL Birnbaum Kenneth D KD

Science (New York, N.Y.) 20211202 6572

Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expa ...[more]

PMID: 34855479

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