Comparison of urine proteome among rat models by intraperitoneal injection with single bacteria and co-injection with two bacteria.

ABSTRACT:

Purpose

To explore and compare urine proteome changes among rat models by intraperitoneal injection with single bacteria and co-injection with two bacteria.

Method

Escherichia coli and Staphylococcus aureus are two common human pathogens. Three rat models were established: (i) the intraperitoneal co-injection of E. coli and S. aureus model (ES model), (ii) intraperitoneal injection of E. coli model (E model), and (iii) intraperitoneal injection of S. aureus model (S model). Urinary proteomes on days 0, 1 and 2 of the three models were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

Results

A total of 111, 34 and 94 differential proteins were identified in the ES model, E model and S model, respectively. Among them, some differential

SUBMITTER: Meng W 

PROVIDER: S-EPMC8719777 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature