Cytoglobin has potent superoxide dismutase function.

ABSTRACT: Cytoglobin (Cygb) was discovered as a novel type of globin that is expressed in mammals; however, its functions remain uncertain. While Cygb protects against oxidant stress, the basis for this is unclear, and the effect of Cygb on superoxide metabolism is unknown. From dose-dependent studies of the effect of Cygb on superoxide catabolism, we identify that Cygb has potent superoxide dismutase (SOD) function. Initial assays using cytochrome c showed that Cygb exhibits a high rate of superoxide dismutation on the order of 10⁸ M^-1 ⋅ s^-1 Spin-trapping studies also demonstrated that the rate of Cygb-mediated superoxide dismutation (1.6 × 10⁸ M^-1 ⋅ s^-1) was only ∼10-fold less than Cu,Zn-SOD. Stopped-flow experiments confirme

SUBMITTER: Zweier JL 

PROVIDER: S-EPMC8719900 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature