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Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection.

ABSTRACT: This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)₃]²⁺ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)₃]²⁺/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.

SUBMITTER: Gutierrez-Galvez L

PROVIDER: S-EPMC8719920 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection.

Gutiérrez-Gálvez Laura L Del Caño Rafael R Menéndez-Luque Iris I García-Nieto Daniel D Rodríguez-Peña Micaela M Luna Mónica M Pineda Teresa T Pariente Félix F García-Mendiola Tania T Lorenzo Encarnación E

Talanta 20220101

This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)<sub>3</sub>]<sup>2+</sup> ano ...[more]

PMID: 34998140

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