Rapid and sensitive detection of SARS-CoV-2 variants in nasopharyngeal swabs and wastewaters.

ABSTRACT: The SARS-CoV-2 virus is continuously evolving, with appearance of new variants characterized by multiple genomic mutations, some of which can affect functional properties, including infectivity, interactions with host immunity, and disease severity. The rapid spread of new SARS-CoV-2 variants has highlighted the urgency to trace the virus evolution, to help limit its diffusion, and to assess effectiveness of containment strategies. We propose here a PCR-based rapid, sensitive and low-cost allelic discrimination assay panel for the identification of SARS-CoV-2 genotypes, useful for detection in different sample types, such as nasopharyngeal swabs and wastewater. The tests carried out demonstrate that this in-house assay, whose results were confirmed by SARS-CoV-2 whole-genome sequencing, ca

SUBMITTER: D'Agostino Y 

PROVIDER: S-EPMC8719921 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature