Production of galactosylated complex-type N-glycans in glycoengineered Saccharomyces cerevisiae.

ABSTRACT: N-glycosylation is an important posttranslational modification affecting the properties and quality of therapeutic proteins. Glycoengineering in yeast aims to produce proteins carrying human-compatible glycosylation, enabling the production of therapeutic proteins in yeasts. In this work, we demonstrate further development and characterization of a glycoengineering strategy in a Saccharomyces cerevisiae Δalg3 Δalg11 strain where a truncated Man₃GlcNAc₂ glycan precursor is formed due to a disrupted lipid-linked oligosaccharide synthesis pathway. We produced galactosylated complex-type and hybrid-like N-glycans by expressing a human galactosyltransferase fusion protein both with and without a UDP-glucose 4-epimerase domain from Schizosaccharomyces pombe. Our results sho

SUBMITTER: Piirainen MA 

PROVIDER: S-EPMC8720083 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature