Identification and validation of reference genes for qRT-PCR based studies in horse gram (Macrotyloma uniflorum).

ABSTRACT: The quantitative real-time polymerase chain reaction (qRT-PCR) is the most sensitive and commonly used technique for gene expression studies in biological systems. However, the reliability of qRT-PCR results depends on the selection of reference gene(s) for data normalization. Horse gram (Macrotyloma uniflorum) is an important legume crop on which several molecular studies have been reported. However, the stability of reference genes has not been evaluated. In the present study, nine candidate reference genes were identified from horse gram RNA-seq data and evaluated in two horse gram genotypes, HPK4 and HPKM317 under six abiotic stresses viz. cold, drought, salinity, heat, abscisic acid and methyl viologen-induced oxidative stress. The results were evaluated using geNorm, Be

SUBMITTER: Sinha R 

PROVIDER: S-EPMC8720121 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature