Multiomic analysis reveals decidual-specific transcriptional programing of MAIT cells.

ABSTRACT:

Problem

Mucosal-Associated Invariant T (MAIT) cells have been recently identified at the maternal-fetal interface. However, transcriptional programming of decidual MAIT cells in pregnancy remains poorly understood.

Method of study

We employed a multiomic approach to address this question. Mononuclear cells from the decidua basalis and parietalis, and control PBMCs, were analyzed via flow cytometry to investigate MAIT cells in the decidua and assess their transcription factor expression. In a separate study, both decidual and matched peripheral MAIT cells were analyzed using Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) coupled with gene expression analysis. Lastly, decidual MAIT cells were stimulated with E.coli and expression of MR1 by antigen pr

SUBMITTER: Vazquez J 

PROVIDER: S-EPMC8720468 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature