LncRNA and mRNA expression associated with myasthenia gravis in patients with thymoma.

ABSTRACT:

Objective

Pathological alterations of the thymus are observed in the majority of patients with myasthenia gravis (MG). To explore the potential mechanisms of these alterations, we performed a transcriptome analysis and measured co-expression of aberrant long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs).

Methods

RNA was extracted from eight patients with thymoma, five of whom had MG. Transcriptome profiles were acquired through mRNA and lncRNA microarray analysis. Quantitative reverse transcription polymerase chain reaction was used to verify the results of the microarray analysis. LncRNAs co-expressed with mRNA were analyzed with Pearson's coefficient. Next, cis-regulated and trans-regulated target genes were predicted. The functions of aberrant lncRNAs were explored

SUBMITTER: Ke J 

PROVIDER: S-EPMC8720629 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature