Impact of Sex Hormones on Macrophage Responses to Coxiella burnetii.

ABSTRACT:

Introduction

Q fever, a zoonosis caused by Coxiella burnetii, affects more males than females despite a similar level of exposure. A protective role of estradiol has been reported in mice, suggesting that sex hormones are involved in C. burnetii infection. We wondered whether the responses of monocytes and monocyte-derived macrophages (MDMs) to C. burnetii are influenced by sex hormones.

Materials and methods

The bacterial intracellular fate in monocytes was studied using quantitative PCR, and monocyte cytokine production in response to C. burnetii was assessed using qRT-PCR and immunoassays. Before infection, MDMs from males and females were incubated with testosterone and estradiol, respectively.

Results

Bacterial uptake and persistence w

SUBMITTER: Gay L 

PROVIDER: S-EPMC8720845 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature