Adaptive Immune Response Signaling Is Suppressed in Ly6C^high Monocyte but Upregulated in Monocyte Subsets of ApoE^-/- Mice - Functional Implication in Atherosclerosis.

ABSTRACT:

Rationale

Inflammatory monocyte (MC) subset differentiation is a major feature in tissue inflammatory and atherosclerosis. The underlying molecular mechanism remains unclear.

Objective

This study aims to explore molecule targets and signaling which determinate immunological features in MC subsets.

Methods and results

Blood Ly6C^high and Ly6C^low MC subsets from control and ApoE^-/- mice were isolated by flow cytometry sorting and subjected for bulk high-throughput RNA-sequencing. Intensive bioinformatic studies were performed by analyzing transcriptome through four pairs of comparisons: A) Ly6C^high vs Ly6C^low in control mice; B) Ly6C^high vs Ly6C^low in ApoE^-/- mice; C)

SUBMITTER: Yang P 

PROVIDER: S-EPMC8721109 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature