In vitro Modeling of Chicken Cecal Microbiota Ecology and Metabolism Using the PolyFermS Platform.

ABSTRACT: Continuous in vitro fermentation models provide a useful tool for a fast, reproducible, and direct assessment of treatment-related changes in microbiota metabolism and composition independent of the host. In this study, we used the PolyFermS model to mimic the conditions of the chicken cecum and evaluated three nutritive media for in vitro modeling of the chicken cecal microbiota ecology and metabolism. We observed that our model inoculated with immobilized cecal microbiota and fed with a modified Viande Levure medium (mVL-3) reached a high bacterial cell density of up to approximately 10.5 log cells per mL and stable microbiota composition, akin to the host, during 82 days of continuous operation. Relevant bacterial functional groups containing primary fibrolytic (Bactero

SUBMITTER: Asare PT 

PROVIDER: S-EPMC8721126 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature