Autophagy Is Involved in Mesenchymal Stem Cell Death in Coculture with Chondrocytes.

ABSTRACT:

Objective

Cartilage formation is stimulated in mixtures of chondrocytes and human adipose-derived mesenchymal stromal cells (MSCs) both in vitro and in vivo. During coculture, human MSCs perish. The goal of this study is to elucidate the mechanism by which adipose tissue-derived MSC cell death occurs in the presence of chondrocytes.

Methods

Human primary chondrocytes were cocultured with human MSCs derived from 3 donors. The cells were cultured in monoculture or coculture (20% chondrocytes and 80% MSCs) in pellets (200,000 cells/pellet) for 7 days in chondrocyte proliferation media in hypoxia (2% O₂). RNA sequencing was performed to assess for differences in gene expression between monocultures or coculture. Immune fluorescence assays were performed

SUBMITTER: Paggi CA 

PROVIDER: S-EPMC8721613 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature