Creating Virtual Hematoxylin and Eosin Images using Samples Imaged on a Commercial CODEX Platform.

ABSTRACT: Multiparametric fluorescence imaging through CODEX allows the simultaneous imaging of many biomarkers in a single tissue section. While the digital fluorescence data thus obtained can provide highly specific characterizations of individual cells and microenvironments, the images obtained are different from those usually interpreted by pathologists (i.e., hematoxylin and eosin [H&E] slides and 3,3'-diaminobenzidine-stained immunohistochemistry slides). Having the fluorescence data plus coregistered H&E or similar data could facilitate the adoption of multiparametric imaging into regular workflows, as well as facilitate the transfer of algorithms and machine learning previously developed around H&E slides. Since commercial CODEX instruments do not produce H&E-like images by themselves, we de

SUBMITTER: Simonson PD 

PROVIDER: S-EPMC8721868 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature