Ketogenic diet ameliorates lipid dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1.

ABSTRACT:

Background

Previous reports implied a possible link between PES1 and lipid metabolism. However, the role of PES1 in regulating T2DM related lipid metabolism and the effect of ketogenic diet (KD) on PES1 have not been reported. The aim of present study is to explore the role of PES1 in effects of KD on diabetic mice and its mediated mechanism.

Methods

Male C57BL/6J and KKA^y mice were fed with standard diet (SD) and KD, respectively. Simultaneously, McArdle 7777 cells were treated by β-hydroxybutyric acid (β-HB), Pes1 siRNA or Pes1 overexpression plasmid, respectively. Additionally, liver-conditional knockout (CKO) of Pes1 in vivo was applied.

Results

Hepatic PES1 expression in diabetic mice was markedly increased, which was suppressed by KD feeding with an a

SUBMITTER: Zhou J 

PROVIDER: S-EPMC8722053 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature