N⁴-acetyldeoxycytosine DNA modification marks euchromatin regions in Arabidopsis thaliana.

ABSTRACT:

Background

Direct analogs of chemically modified bases that carry important epigenetic information, such as 5-methylcytosine (m5C)/5-methyldeoxycytosine (5mC), 5-hydroxymethylcytosine (hm5C)/5-hydroxymethyldeoxycytosine (5hmC), and N⁶-methyladenosine (m6A)/N⁶-methyldeoxyadenosine (6mA), are detected in both RNA and DNA, respectively. The modified base N⁴-acetylcytosine (ac4C) is well studied in RNAs, but its presence and epigenetic roles in cellular DNA have not been explored.

Results

Here, we demonstrate the existence of N⁴-acetyldeoxycytosine (4acC) in genomic DNA of Arabidopsis with multiple detection methods. Genome-wide profiling of 4acC modification reveals that 4acC peaks are mostly distributed in euchromatin regions and pre

SUBMITTER: Wang S 

PROVIDER: S-EPMC8722123 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature