CircCDYL2 promotes trastuzumab resistance via sustaining HER2 downstream signaling in breast cancer.

ABSTRACT:

Background

Approximate 25% HER2-positive (HER2⁺) breast cancer (BC) patients treated with trastuzumab recurred rapidly. However, the mechanisms underlying trastuzumab resistance remained largely unclear.

Methods

Trastuzumab-resistant associated circRNAs were identified by circRNAs high-throughput screen and qRT-PCR in HER2⁺ breast cancer tissues with different trastuzumab response. The biological roles of trastuzumab-resistant associated circRNAs were detected by cell vitality assay, colony formation assay, Edu assay, patient-derived xenograft (PDX) models and orthotopic animal models. For mechanisms research, the co-immunoprecipitation, Western blot, immunofluorescence, and pull down assays confirmed the relevant mechanisms of circRNA and binding prote

SUBMITTER: Ling Y 

PROVIDER: S-EPMC8722291 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature