Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells.

ABSTRACT: The three-dimensional configuration of the genome ensures cell type-specific gene expression profiles by placing genes and regulatory elements in close spatial proximity. Here, we used in situ high-throughput chromosome conformation (in situ Hi-C), RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) to characterize the high-order chromatin structure signature of female germline stem cells (FGSCs) and identify its regulating key factor based on the data-driven of multiple omics data. By comparison with pluripotent stem cells (PSCs), adult stem cells (ASCs), and somatic cells at three major levels of chromatin architecture, A/B compartments, topologically associating domains, and chromatin loops, the chromatin architecture of FGSCs was most similar to that of oth

SUBMITTER: Tian GG 

PROVIDER: S-EPMC8724064 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature