EHooke: A tool for automated image analysis of spherical bacteria based on cell cycle progression.

ABSTRACT: Fluorescence microscopy is a critical tool for cell biology studies on bacterial cell division and morphogenesis. Because the analysis of fluorescence microscopy images evolved beyond initial qualitative studies, numerous images analysis tools were developed to extract quantitative parameters on cell morphology and organization. To understand cellular processes required for bacterial growth and division, it is particularly important to perform such analysis in the context of cell cycle progression. However, manual assignment of cell cycle stages is laborious and prone to user bias. Although cell elongation can be used as a proxy for cell cycle progression in rod-shaped or ovoid bacteria, that is not the case for cocci, such as Staphylococcus aureus. Here, we describe eHooke, an imag

SUBMITTER: Saraiva BM 

PROVIDER: S-EPMC8724265 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature