Adaptive laboratory evolution triggers pathogen-dependent broad-spectrum antimicrobial potency in Streptomyces.

ABSTRACT:

Background

In the present study, adaptive laboratory evolution was used to stimulate antibiotic production in a Streptomyces strain JB140 (wild-type) exhibiting very little antimicrobial activity against bacterial pathogens. The seven different competition experiments utilized three serial passages (3 cycles of adaptation-selection of 15 days each) in which Streptomyces strain (wild-type) was challenged repeatedly to one (bi-culture) or two (tri-culture) or three (quadri-culture) target pathogens. The study demonstrates a simple laboratory model to study the adaptive potential of evolved phenotypes and genotypes in Streptomyces to induce antibiotic production.

Results

Competition experiments resulted in the evolution of the wild-type Streptomyces strain JB140 into the seven

SUBMITTER: Harwani D 

PROVIDER: S-EPMC8724467 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature